Coding

Part:BBa_K4649003:Design

Designed by: Sophia Eowyn Hsu   Group: iGEM23_EastCoastBioCrew   (2023-09-09)


pstA codon optimized for Rhizobium tropici


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 487
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 487
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 140
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 487
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 487
    Illegal NgoMIV site found at 68
    Illegal NgoMIV site found at 250
    Illegal NgoMIV site found at 310
    Illegal NgoMIV site found at 808
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 670
    Illegal SapI site found at 189


Design Notes

Codon optimized for Rhizobium tropici


Source

Escherichia coli strain K12 genome (UniProt accession: P07654)

References